Sample prep

Sample prep

34 items

Ligation Sequencing Kit - PromethION SQK-LSK109P

A versatile sequencing kit optimised for throughput - for PromethION Sequencing device only

  • Preparation time: 60
  • Throughput: 10-15+ Gb in 6 hours, 40+ Gb in 48 hours
  • Read length: = fragment length
  • Input amount: 1000 ng dsDNA

Ligation Sequencing Kit SQK-LSK110

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109

  • Preparation time: 60 minutes
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
  • Read length: = fragment length
  • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Flow Cell Wash Kit EXP-WSH004

The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

    VolTRAX Multiplex Kit VSK-VMK002

    A simple and rapid library preparation method for genomic DNA on the VolTRAX, with barcoding for up to 10 gDNA samples.

    This product has a lead time of 6 weeks.

    • Preparation time: 10 minutes
    • Throughput: 10 – 20 Gb in 48 hours
    • Read length: Random distribution, dependent on input fragment length
    • Input amount: 55 ng dsDNA

    VolTRAX Cartridge Pack VCT-V2002D

    The VolTRAX V2 Cartridge Pack contains three disposable cartridges that interface with the VolTRAX V2 device.

    • Preparation time: 5 minutes
    • Throughput: 10 - 20 GB in 48 hours
    • Read length: Random distribution, dependent on input fragment length
    • Input amount: 400 ng dsDNA

    Ligation Sequencing Kit SQK-LSK109

    A versatile sequencing kit optimised for throughput

     Includes a Flow Cell Priming Kit
    • Preparation time: 60
    • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
    • Read length: = fragment length
    • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

    Rapid Sequencing Kit SQK-RAD004

    A simple and rapid library preparation method for genomic DNA.

    • Preparation time: 10
    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: Random distribution, dependent on input fragment length
    • Input amount: 400 ng HMW gDNA

    PCR Sequencing Kit SQK-PSK004

    This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

    • Preparation time: PCR + 60
    • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION
    • Read length: = fragment length post-PCR
    • Input amount: 100 ng dsDNA

    Rapid PCR Barcoding Kit SQK-RPB004

    Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

    • Preparation time: PCR + 15
    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: ~2 kb
    • Input amount: 1-5 ng gDNA

    PCR Barcoding Kit SQK-PBK004

    This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR

    • Preparation time: PCR + 60
    • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION
    • Read length: = fragment length post-PCR
    • Input amount: 100 ng dsDNA

    Rapid Barcoding Kit SQK-RBK004

    A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples

    • Preparation time: 10
    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: Random distribution, dependent on input fragment length
    • Input amount: 400 ng HMW gDNA

    Direct cDNA Sequencing Kit SQK-DCS109

    Identification and quantification of full-length transcripts without PCR bias

    • Preparation time: 270
    • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
    • Read length: Enriched for full-length cDNA
    • Input amount: 100 ng poly-A+ RNA

    PCR-cDNA Sequencing Kit SQK-PCS109

    Identification and quantification of full-length transcripts with highest throughput

    • Preparation time: 165
    • Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
    • Read length: Enriched for full-length cDNA
    • Input amount: 1 ng poly-A+ or 50 ng total RNA

    Direct RNA Sequencing Kit SQK-RNA002

    Prepare RNA for direct RNA nanopore sequencing

    • Preparation time: 115
    • Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION
    • Read length: = RNA length
    • Input amount: 500 ng RNA (poly-A+)

    Field Sequencing Kit SQK-LRK001

    Cold chain-free library preparation for ultimate portability.

    • Preparation time: 10
    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: Random distribution, dependent on input fragment length
    • Input amount: 400 ng dsDNA

    16S Barcoding Kit 1-24 SQK-16S024

    This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 24 barcoded samples at a time

    • Preparation time: PCR + 10
    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: = full-length 16S gene (~1.5kb)
    • Input amount: 10 ng gDNA

    Ligation Sequencing Kit XL SQK-LSK109-XL

    A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

    • Preparation time: 110
    • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
    • Read length: = fragment length
    • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

    PCR-cDNA Barcoding Kit SQK-PCB109

    Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

    • Preparation time: 165
    • Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
    • Read length: Enriched for full-length cDNA during PCR
    • Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA

    Cas9 Sequencing Kit SQK-CS9109

    Targeted sequencing of genomic regions using CRISPR-Cas9

    • Preparation time: 110
    • Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: = fragment length
    • Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)

    16S Barcoding Kit SQK-RAB204

    This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time

    • Preparation time: PCR + 10
    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
    • Read length: = full-length 16S gene (~1.5kb)
    • Input amount: 10 ng gDNA

    Control Expansion EXP-CTL001

    Lambda DNA (LMD). Pack of six vials

      PCR Expansion EXP-PCA001

      PCR Primers (PRM) and PCR Adapters (PCA)

        RAP Top-Up Kit EXP-RAP001

        Rapid 1D sequencing adapters (RAP)

          PCR Barcoding Expansion 1-96 EXP-PBC096

          The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode

          • Preparation time: 150'
          • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
          • Read length: = to fragment length post PCR
          • Input amount: 1000 ng dsDNA

          PCR Barcoding Expansion 1-12 EXP-PBC001

          The PCR Barcoding Expansion 1-12 (EXP-PBC001) allows the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 12 unique barcodes, sufficient for 6 reactions

          • Preparation time: 150'
          • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
          • Read length: = to fragment length post PCR
          • Input amount: 1000 ng dsDNA

          Native Barcoding Expansion 13-24 (PCR-free) EXP-NBD114

          A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

          • Preparation time: 80'
          • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
          • Read length: = to fragement length
          • Input amount: 1000 ng dsDNA

          Native Barcoding Expansion 1-12 (PCR-free) EXP-NBD104

          A versatile method of preparing barcoded sequencing libraries for applications where high throughput is required

          • Preparation time: 80'
          • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours
          • Read length: = to fragement length
          • Input amount: 1000 ng dsDNA

          Flow Cell Wash Kit EXP-WSH003

          The Wash Kit provides the reagents required to wash and reuse MinION™ and PromethION™ Flow Cells.

            SFB Expansion EXP-SFB001

            Short Fragment Buffer (SFB)

              Flow Cell Priming Kit XL EXP-FLP002-XL

              Flow Cell Priming Kit XL

              This product has a lead time of 3 weeks.

                Sequencing Auxiliary Vials EXP-AUX001

                The Sequencing Auxiliary Vials contain Sequencing Buffer (SQB), Elution Buffer (EB) and Loading Beads (LB), sufficient for 12 MinION/GridION or PromethION flow cells.

                  Flow Cell Priming Kit EXP-FLP002

                  Flow Cell Priming Kit

                    Native Barcoding Expansion 96 EXP-NBD196

                    A versatile method of preparing a high number of barcoded sequencing libraries for applications where high throughput is required

                    • Preparation time: PCR + 10
                    • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
                    • Read length: = full-length 16S gene (~1.5kb)
                    • Input amount: 10 ng gDNA

                    VolTRAX Sequencing Kit VSK-VSK002

                    A simple and rapid library preparation method for genomic DNA on the VolTRAX

                    • Preparation time: 10 minutes
                    • Throughput: 10 – 20 Gb in 48 hours
                    • Read length: Random distribution, dependent on input fragment length
                    • Input amount: 475 ng dsDNA