A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109

• Preparation time: 60 minutes
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

A versatile sequencing kit optimised for throughput

• Preparation time: 60
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

A simple and rapid library preparation method for genomic DNA.

• Preparation time: 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

• Preparation time: PCR + 60
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

• Preparation time: PCR + 15
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: ~2 kb
• Input amount: 1-5 ng gDNA

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR

• Preparation time: PCR + 60
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION
• Read length: = fragment length post-PCR
• Input amount: 100 ng dsDNA

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples

• Preparation time: 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng HMW gDNA

Identification and quantification of full-length transcripts without PCR bias

• Preparation time: 270
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
• Read length: Enriched for full-length cDNA
• Input amount: 100 ng poly-A+ RNA

Identification and quantification of full-length transcripts with highest throughput

• Preparation time: 165
• Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
• Read length: Enriched for full-length cDNA
• Input amount: 1 ng poly-A+ or 50 ng total RNA

Prepare RNA for direct RNA nanopore sequencing

• Preparation time: 115
• Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION
• Read length: = RNA length
• Input amount: 500 ng RNA (poly-A+)

Cold chain-free library preparation for ultimate portability.

• Preparation time: 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: Random distribution, dependent on input fragment length
• Input amount: 400 ng dsDNA

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 24 barcoded samples at a time

• Preparation time: PCR + 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

• Preparation time: 110
• Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
• Read length: = fragment length
• Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

• Preparation time: 165
• Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA

Targeted sequencing of genomic regions using CRISPR-Cas9

• Preparation time: 110
• Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION
• Read length: = fragment length
• Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time

• Preparation time: PCR + 10
• Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
• Read length: = full-length 16S gene (~1.5kb)
• Input amount: 10 ng gDNA