Multiplexing
PCR-Free
Sample type
16 items

Ligation Sequencing Kit SQK-LSK110

A versatile sequencing kit optimised for throughput, and long reads, and processing singleplex samples. For multiplexing samples using ligation-based sample preparation, please use SQK-LSK109

  • Preparation time: 60 minutes
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
  • Read length: = fragment length
  • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Ligation Sequencing Kit SQK-LSK109

A versatile sequencing kit optimised for throughput

 Includes a Flow Cell Priming Kit
  • Preparation time: 60
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
  • Read length: = fragment length
  • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

Rapid Sequencing Kit SQK-RAD004

A simple and rapid library preparation method for genomic DNA.

  • Preparation time: 10
  • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 400 ng HMW gDNA

PCR Sequencing Kit SQK-PSK004

This kit prepares genomic DNA libraries from as little as 100 ng DNA, using PCR.

  • Preparation time: PCR + 60
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION
  • Read length: = fragment length post-PCR
  • Input amount: 100 ng dsDNA

Rapid PCR Barcoding Kit SQK-RPB004

Rapid and simple method of preparing up to 12 barcoded gDNA samples for sequencing from 1-5 ng using PCR.

  • Preparation time: PCR + 15
  • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: ~2 kb
  • Input amount: 1-5 ng gDNA

PCR Barcoding Kit SQK-PBK004

This kit prepares up to 12 barcoded gDNA libraries from as little as 100 ng DNA, using PCR

  • Preparation time: PCR + 60
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION
  • Read length: = fragment length post-PCR
  • Input amount: 100 ng dsDNA

Rapid Barcoding Kit SQK-RBK004

A simple and rapid library preparation method, with barcoding for up to 12 genomic DNA samples

  • Preparation time: 10
  • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 400 ng HMW gDNA

Direct cDNA Sequencing Kit SQK-DCS109

Identification and quantification of full-length transcripts without PCR bias

  • Preparation time: 270
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
  • Read length: Enriched for full-length cDNA
  • Input amount: 100 ng poly-A+ RNA

PCR-cDNA Sequencing Kit SQK-PCS109

Identification and quantification of full-length transcripts with highest throughput

  • Preparation time: 165
  • Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
  • Read length: Enriched for full-length cDNA
  • Input amount: 1 ng poly-A+ or 50 ng total RNA

Direct RNA Sequencing Kit SQK-RNA002

Prepare RNA for direct RNA nanopore sequencing

  • Preparation time: 115
  • Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION; <5 Gb in 6 hours, 5-20 Gb in 48 hours per flow cell on PromethION
  • Read length: = RNA length
  • Input amount: 500 ng RNA (poly-A+)

Field Sequencing Kit SQK-LRK001

Cold chain-free library preparation for ultimate portability.

  • Preparation time: 10
  • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: Random distribution, dependent on input fragment length
  • Input amount: 400 ng dsDNA

16S Barcoding Kit 1-24 SQK-16S024

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 24 barcoded samples at a time

  • Preparation time: PCR + 10
  • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: = full-length 16S gene (~1.5kb)
  • Input amount: 10 ng gDNA

Ligation Sequencing Kit XL SQK-LSK109-XL

A versatile sequencing kit optimised for throughput, long reads, and processing multiple samples simultaneously.

  • Preparation time: 110
  • Throughput: 2-3+ Gb in 6 hours, 8+ Gb in 48 hours per flow cell on MinION/GridION; 10-15+ Gb in 6 hours, 40+ Gb in 48 hours per flow cell on PromethION
  • Read length: = fragment length
  • Input amount: 1000 ng high molecular weight dsDNA 100+ ng DNA if performing fragmentation or PCR

PCR-cDNA Barcoding Kit SQK-PCB109

Identification and quantification of full-length transcripts with highest throughput, with barcoding for ≤12 samples

  • Preparation time: 165
  • Throughput: 7-12+ million full-length mapped reads in 48 hours per flow cell on MinION/GridION; 50+ million mapped reads in 48 hours per flow cell on PromethION
  • Read length: Enriched for full-length cDNA during PCR
  • Input amount: 1 ng poly-A+ RNA, or 50 ng total RNA

Cas9 Sequencing Kit SQK-CS9109

Targeted sequencing of genomic regions using CRISPR-Cas9

  • Preparation time: 110
  • Throughput: <1 Gb in 6 hours, 1-4 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: = fragment length
  • Input amount: 1–10 µg of double-stranded DNA (5 µg recommended)

16S Barcoding Kit SQK-RAB204

This kit allows the user to carry out rapid 16S sequencing for bacterial identification on up to 12 barcoded samples at a time

  • Preparation time: PCR + 10
  • Throughput: 1-2 Gb in 6 hours, 4-8 Gb in 48 hours per flow cell on MinION/GridION
  • Read length: = full-length 16S gene (~1.5kb)
  • Input amount: 10 ng gDNA