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PCR Barcoding Expansion 1-96
EXP-PBC096

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode.

Information

The PCR Barcoding Expansion 1-96 (EXP-PBC096) is designed to allow the pooling and running together of multiple sequencing libraries. The kit includes adapters and forward and reverse PCR primers with the same barcode in both forward and reverse primers. There are 96 unique barcodes in the kit. There are 96 unique barcodes and enough of the required adapters to make 10 libraries per barcode. The barcodes have been carefully designed and extensively purified to minimise the opportunity for cross-talk.

There are currently two protocols for this kit which are provided as examples of how the PCR Barcoding Expansion 1-96 could be applied for genomic and amplicon libraries. Please note that if you are barcoding cDNA or amplicons, these have to be pre-made in advance. These protocols are open for adaptation and optimisation for specific experimental requirements.

This kit is used with the Ligation Sequencing Kit for library preparation. Store at -20°C.

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Workflow

EXPPBC v1

What's in the box

There are 96 unique barcodes in the kit. Each kit provides material sufficient for 10 reactions of each barcode.

96 barcode kit

NameNo. of platesFill volume per well (µl)
PCR Primer mix124
Barcode adapter plate1240

3rd party materials

In addition to the equipment and consumables used with the Ligation Sequencing Kit:

  • LongAmp Taq 2X Master Mix (e.g. NEB cat # M0287)
  • Magnet for 96-well plates (for 96 barcodes)
  • Multi-channel pipette (for 96 barcodes)
  • 0.2 ml 96-well PCR plates and seals (for 96 barcodes)

Compatibility

The PCR Barcoding Expansion 1-96 can be used together with:

Kits

  • Ligation Sequencing Kit (SQK-LSK110)
  • Ligation Sequencing Kit (SQK-LSK109)
  • Flow Cell Wash Kit (EXP-WSH004)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • MinION Mk1B
  • MinION Mk1C
  • GridION