A Flongle Flow Cell (R9.4.1) is a consumable used with the Flongle adapter and MinION and GridION X5 devices. It contains the proprietary sensor array and R9.4.1 nanopores suitable for all 1D experiments.
How it works:
A user simply adds their prepared library to the flow cell and starts their sequencing experiment.
Data is accumulated over time as DNA or RNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis.
As data is streamed in real-time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~24 hours (under optimal conditions).
Shipping and logistics:
Flongle Flow Cells are packed individually; however, bulk order options are available. All orders can be spilt for delivery to best suit your needs.
Flow cells and kits are shipped together in a temperature-controlled shipping box with gel ice packs. Ice packs are stored at 2-8° C, and -20° C ice packs are added to the top of the box (but not in direct contact with product) to maintain the integrity of the shipment.
The packaging is designed to protect the flow cells from freezing. Sequencing kits are stable at room temperature for up to seven days, so users should not be concerned about their products should they arrive at ambient temperature.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information can be tracked in the Store.
Storage and stability:
Please store your flow cells as follows:
- Flow cells can be stored, unopened, at 2-8° C for two weeks.
We recommend you check your flow cell before preparing your DNA/RNA library as part of the warranty process.
Flongle Flow Cells are provided in a sealed pouch and are shipped at constant temperature.
It is recommended that they are kept, unopened, at 2-8° C.
Prior to use, the flow cell can be checked by performing a “Flow Cell Check” script using MinKNOW (the MinION and GridION device software). The Flow Cell Check will report the number of nanopores available for sequencing.
Once the Flow Cell Check has been performed satisfactorily, the flow cell is ready to be used for experiments. The exact protocol and reagent kits used will depend on the experiment being run.
Safety and legal
Safety data sheet for Flongle Flow Cell buffer
Customers are required to perform a flow cell check prior to starting their sequencing experiment. Oxford Nanopore Technologies will replace any flow cell with fewer than 60 nanopores, when the result is reported within two days of performing the flow cell check, and when the storage recommendations have been followed. We deem the useful life of this product to be 4 weeks from receipt by the customer.
There is a 1 year contract length on all consumables purchases.
What's in the box
Flongle Flow Cells are shipped in a sealed pouch to maintain the environment for the flow cell chemistry.
Flongle R9.4.1 flow cells have been tested with the following 1D sequencing kits:
- Ligation Sequencing Kit (SQK-LSK109)
- Rapid Sequencing Kit (SQK-RAD004)
- Rapid Barcoding Kit (SQK-RBK004)
And the Native Barcoding Expansions:
- Native Barcoding Expansion 1-12 (EXP-NBD104)
- Native Barcoding Expansion 13-24 (EXP-NBD114)
R9.4.1 flow cells are not recommended for use with 1D^2 chemistry:
- 1D^2 Sequencing Kit (SQK-LSK309)