Information
This kit is recommended for users who:
- are working in the field, with limited access to cold storage and laboratory equipment.
The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
The Field Sequencing Kit features:
| Feature | Property |
|---|
| Preparation time | 10 minutes |
| Input requirement | 400 ng of high molecular weight gDNA (<30 kb) |
| PCR Required | No |
| Fragmentation | Transposase-based |
| Read length | Random distribution, dependent on input fragment length |
| Read type produced | 1D |
| Typical throughput* | ••• |
| Associated protocols | Field Sequencing Kit |
| Multiplexing options | Flow Cell Wash Kit |
| Pack size | 6 reactions |
| Stability | Shipped at 2–8° C and Long-term storage: up to 30° C for one month unopened, or at 2–8° C for three months unopened. The Flow Cell Priming Kit shipped with the Field Sequencing Kit can be stored in the same conditions. |
The kit is optimised to be stable at ambient temperatures for extended periods, with many of the reagents supplied in lyophilized form. The stability of the components in this kit enables it to be highly portable, making it ideal for use in the field where access to cold storage may be limited.
Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.
Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.
The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.
Further considerations:
Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.
Workflow
The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.
What's in the box
The Field Sequencing Kit contains sufficient reagents to generate six sequencing libraries.
The Flow Cell Priming Kit is also supplied.
Compatibility
The Field Sequencing Kit can be used together with:
Kits
- Flow Cell Wash Kit (EXP-WSH003)
Flow cells
Devices
