Field Sequencing Kit
SQK-LRK001

Cold chain-free library preparation for ultimate portability.

 Includes a Flow Cell Priming Kit

Information

This kit is recommended for users who:

  • are working in the field, with limited access to cold storage and laboratory equipment.

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends. The Field Sequencing Kit features:

FeatureProperty
Preparation time10 minutes
Input requirement400 ng of high molecular weight gDNA (<30 kb)
PCR RequiredNo
FragmentationTransposase-based
Read lengthRandom distribution, dependent on input fragment length
Read type produced1D
Typical throughput*•••
Associated protocolsField Sequencing Kit
Multiplexing optionsFlow Cell Wash Kit
Pack size6 reactions
StabilityShipped at 2–8° C and Long-term storage: up to 30° C for one month unopened, or at 2–8° C for three months unopened. The Flow Cell Priming Kit shipped with the Field Sequencing Kit can be stored in the same conditions.

The kit is optimised to be stable at ambient temperatures for extended periods, with many of the reagents supplied in lyophilized form. The stability of the components in this kit enables it to be highly portable, making it ideal for use in the field where access to cold storage may be limited.

LRK001-read-length

Typical read length histogram when preparing an E.coli library with the Rapid Sequencing Kit.

LRK001-throughput Typical throughput achieved with the Rapid Sequencing Kit compared with the Ligation Sequencing Kit.

The workflow is PCR-free and therefore removes PCR bias and retains information about base modifications, which can be analysed using tools developed in the Nanopore Community.

Further considerations:

Oxford Nanopore Technologies deem the useful life of the product to be 3 months from receipt by the customer.

Workflow

The Field Sequencing Kit generates sequencing libraries from extracted gDNA in 10 minutes using a simple 2-step protocol. At the heart of the kit is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

RAD004 v1

What's in the box

The Field Sequencing Kit contains sufficient reagents to generate six sequencing libraries. Field Sequencing Kit contents

The Flow Cell Priming Kit is also supplied.

FLP

3rd party materials

Equipment

  • Method of achieving 80° C
  • P1000 pipette and tips
  • P100 pipette and tips
  • P20 pipette and tips
  • P10 pipette and tips

Compatibility

The Field Sequencing Kit can be used together with:

Kits

  • Flow Cell Wash Kit (EXP-WSH003)

Flow cells

  • FLO-MINSP6
  • FLO-MIN106D

Devices

  • MinION Mk 1B
  • GridION X5